Porrykov D.O., Berdiyeva A.M., Akmammedova M.N.

 Porrykov Dovletmyrat Orazmyrat ogly – Head of Department,

MOLECULAR BIOLOGY AND GENETICS DEPARTMENT,

OGUZ HAN ENGINEERING AND TECHNOLOGY UNIVERSITY OF TURKMENISTAN;

Berdiyeva Akjagul Meredovna – Junior Instructor,

DEPARTMENT OF MEDICAL BIOLOGY AND GENETICS,

STATE MEDICAL UNIVERSITY OF TURKMENISTAN NAMED AFTER MYRAT GARRYEV;

Akmammedova Merjen Nuryagdyyevna – Student,

SPECIALITY: CELL AND MOLECULAR BIOLOGY,

FACULTY OF BIOTECHNOLOGY AND ECOLOGY,

OGUZ HAN ENGINEERING AND TECHNOLOGY UNIVERSITY OF TURKMENISTAN,

ASHGABAT, TURKMENISTAN

Abstract: fruits of blackberry contains high amounts of anthocyanin pigments. Histological stains were obtained from alcoholic extract of blackberry fruit and their tissue staining properties were studied. Positive charges of anthocyanins in the extract of blackberry fruit give their basic dye properties.  This allows it to stain the nucleus and basophilic parts of the cytoplasm. At the basis of staining cellular structure stay donor-acceptor interaction. As a result of our scientific work, a general methodology of preparation of high-quality dye from blackberry fruit was developed. This dye can be widely used in cytological, histological and morphological analyses.

Keywords: blackberry, anthocyanin, histological dye, freeze drier, vacuum drier, thin layer chromatography.

References

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Porrykov D.O., Berdiyeva A.M., Akmammedova M.N. EXTRACTION OF ANTHOCYANINS FROM BLACKBERRY FRUITS (RUBUS ANATOLICUS) AND EXAMINATION OF HISTOLOGICAL DYEING PROPERTIES// Academy. № 2 (73), 2022 - С.{см. журнал}.